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. Author manuscript; available in PMC: 2024 Feb 15.
Published in final edited form as: Biosens Bioelectron. 2022 Nov 24;222:114921. doi: 10.1016/j.bios.2022.114921

Figure 1. Overview of the inflamed endothelium-mimicking microfluidic device for assessment of neutrophil binding to E-selectin under physiologic flow in normoxia and clinically relevant hypoxia in SCD.

Figure 1.

(A) Schematics of the fabrication and protein coating process of the microfluidic device. (B) Schematics of the microfluidic device integrated with the micro-gas exchanger for hypoxia induction. (C) Macroscopic view of the microfluidic device mounted on an inverted microscope for blood testing. Insets: Adherent cells were fixed, permeabilized, and fluorescently labeled with anti-human neutrophil elastase antibody. Scale: 50 μm.