Figure 1.

Overview of DISPA-LFQ using CsoDIAq software for peptide and protein group identification and quantification. (A) Scheme showing our strategy for peptide analysis by DISPA with FAIMS. (B) Schematic of a representative targeted experiment with actual data. Peptides were directly infused into the mass spectrometer over the duration of the experiment. Six FAIMS CVs were selected sequentially, and within each CV, specific m/z regions were selected with Q1 before fragmentation to produce chimeric MS/MS spectra. (C) Overview showing identification and label-free quantification of multiple peptides from one chimeric spectrum using the CsoDIAq software package with the same real data example in (B).