Fig. 2.

Biochemical analysis in serum/plasma from Phospho1 knockout (KO) mice under AAV8‐TNAP‐D₁₀ treatment or AAV8‐GFP as control after 45 and 90 days of injection. Wild‐type (WT) littermates were treated with the AAV8‐TNAP‐D₁₀ vector. (A, B) Body weight. (C, D) Serum alkaline phosphatase activity. (E, F) Plasma PP_i levels. (G, H) Serum calcium concentrations. (I, J) Serum phosphorus concentrations. (K, L) Blood urea nitrogen (BUN) levels in serum. Sample size of the cohorts was as follows: (i) Phospho1 KO: AAV8‐TNAP‐D₁₀ female n = 3, male n = 6; (ii) Phospho1 KO: AAV8‐GFP female n = 5, male n = 3; (iii) WT: AAV8‐TNAP‐D₁₀ female n = 3, male n = 4. Statistical analysis was performed by one‐way ANOVA followed by Tukey's multiple comparison test. *p < 0.05; **p < 0.01; ***p < 0.001. ****p < 0.0001.