Fig. 4.

Micro‐CT analysis of femur from adult hypophosphatasia (HPP) mice and wild‐type (WT) littermates. 2D and 3D micro‐CT images of femurs from (A–D) females and (E–H) males treated with AAV8‐TNAP‐D₁₀ or AAV8‐GFP after 60 days of injection. (I–T) Micro‐CT analysis of bone parameters in the femurs showing reduced cortical bone volume fraction (Ct.BV/TV) associated with a reduced cortical thickness (Ct.Th) and increased marrow area (Ma.Ar) in untreated HPP mice compared with AAV8‐TNAP‐D₁₀ or AAV8‐GFP WT controls. These cortical bone defects were partially rescued after 60 days of AAV8‐TNAP‐D₁₀ treatment. Statistical analysis was performed by one‐way ANOVA followed by Tukey's multiple comparison test. Differences were statistically significant at *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001.