TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rK−mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | 3 |
| Bacteroides distasonis 8503 | Type strain | 14 |
| B. fragilis B16 | Clinical isolate | A. B. Onderdonk |
| B. fragilis B16 mutant 65 | Strain B16 with pNJR6Ω4 insertion in PS A region | This study |
| B. fragilis NCTC 9343 | Type strain, appendix abscess | 14; NCTC a |
| B. fragilis 9343ΔPSA | 9343 mutant with a chromosomal deletion removing wzx-wcfS of the PS A region | This study |
| Plasmids | ||
| pHC79 | Cosmid vector; Apr | 12 |
| pBluescript II SK | Phagemid cloning vector; Apr | Stratagene |
| R751 | Mobilizable plasmid used to move plasmids from E. coli to B. fragilis; IncPβ Tra+ Tpr (Tn402) | 20 |
| pNJR6 | Bacteroides suicide vector; mob+ Tra− Kmr | 28 |
| pNJR6Ω4 | Plasmid pNJR6 containing duplicate copies of Tn4351; Emr (Bacteroides) Kmr (E. coli) mob+ Tra− | 28 |
| pLEC12 | Junctional clone of B. fragilis B16 CE3-negative mutant containing a portion of pNJR6Ω4 and several kb of B. fragilis B16 DNA; Kmr | This study |
| pLEC15 | B. fragilis 9343 gene bank clone in pHC79; Apr | This study |
| pLEC15.1 | 4.3-kb EcoRl subclone of pLEC15 in pBluescript; Apr | This study |
| pLEC21 | B. fragilis 9343 gene bank clone in pHC79; Apr | This study |
| pLEC21.1–pLEC21.4 | Subclones of pLEC21 in pBluescript; Apr | This study |
| pMJC15 | Plasmid pNJR6 containing 4.4 and 4.3 kb of the left and right flanks of the 9343 PS A locus; respectively; mob+ Tra−Kmr | This study |
a
National Collection of Type Cultures.