TABLE 2.

Primers and thermal cycler programs used for the PCRs in this study

PCR	Primer	Primer sequence (5′ → 3′)	Primer 5′ positiona	Gene targeted	Programb
1	C464	ACACATATCACTTCCGATGCC	848	orf1	94°C, 30 s; 59°C, 30 s; 72°C, 90 s
	C473	AGAAAACTCCTGGTCCTTCTTTG	2484	upaY
2	C464	ACACATATCACTTCCGATGCC	848	orf1	94°C, 30 s, 59°C, 30 s, 72°C, 90 s
	C444	GTTGACGGAAATGATCGGTATAG	3056	upaZ
2A	C396	TGCTGATGATATTTCCATGCC	10686	wcfQ	94°C, 30 s, 59°C, 30 s, 72°C, 120 s
	C507	CAGAGTAATGGTAAAATCGGGAG	13648	pdiA
3	C432	TAACACGATAGGAGTTGCATGG	3881	wzx	94°C, 30 s, 59°C, 30 s, 72°C, 90 s
	C393	ACATTGAGAAATACTCGTCCACC	6366	wcfN
3A	C379	GGCTGAAGTTGTTTGGAAGTTG	10270	wcfP	94°C, 30 s, 59°C, 30 s, 72°C, 120 s
	C507	CAGAGTAATGGTAAAATCGGGAG	13648	pdiA
4	C202	GATATTTGGTCACATTTGGGG	7814	wzy	94°C, 30 s, 59°C, 30 s, 72°C, 90 s
	C349	GAATTCAGCAGCTTTGTATCCAC	9757	wcfP
6A	C510	AGATCCGAGAATAACTCGTACC	12716	wcfS	94°C, 30 s, 58°C, 30 s, 72°C, 60 s
	C507	CAGAGTAATGGTAAAATCGGGAG	13648	pdiA
7	C505	AATGTCCTGATAGACCGGGAG	13940	pdiA	94°C, 30 s, 59°C, 30 s, 72°C, 90 s
	C500	CAAGGCCGAAATGATAAAAGAG	16081	mtfA
8	C429	CACTCTTTGGGAGCACGTTAC	11753	wcfR	94°C, 30 s, 58°C, 30 s, 72°C, 90 s
	C507	CAGAGTAATGGTAAAATCGGGAG	13648	pdiA
EPCR	EPCR-A3	CTTTTGCCGTATTGGACCATCTTCCCGCTTCTC	3219	upaZ	94°C, 30 s, 68°C, 20 min
	EPCR-A4	GGAACAATCACTTCATCGCCCTCCTTCACTCCC	11472	wcfR

^aIn base pairs. 

^bAll programs had an initial segment at 94°C for 2 min and were run for 30 cycles.