Table 1.
Mutations in the penicillin-resistant in vitro-evolved populations of S. pneumoniae
| Population | Position | Mutationa | Gene | Encoded protein description |
|---|---|---|---|---|
| T4EP1 | 308,636 | Y586S (TAT→TCT) | SP_0336 | PBP2X |
| 324,228 | P80L (CCT→CTT) | SP_0350 | Cps4E | |
| 1,280,284 | F218L (TTT→CTT) | SP_1358 | ABC transporter, ATP-binding/permease protein | |
| 1,442,712 | A430S (GCG→TCG) | SP_1530 | MurE | |
| 2,125,225 | A586E (GCG→GAG) | SP_2205 | DHH subfamily 1 protein | |
| T4EP2 | 308,636 | Y586S (TAT→TCT) | SP_0336 | PBP2X |
| 324,228 | P80L (CCT→CTT) | SP_0350 | Cps4E | |
| 1,280,284 | F218L (TTT→CTT) | SP_1358 | ABC transporter, ATP-binding/permease protein | |
| 1,442,712 | A430S (GCG→TCG) | SP_1530 | MurE | |
| 2,125,225 | A586E (GCG→GAG) | SP_2205 | DHH subfamily 1 protein | |
| T4EP3 | 308,636 | Y586S (TAT→TCT) | SP_0336 | PBP2X |
| 324,228 | P80L (CCT→CTT) | SP_0350 | Cps4E | |
| 1,442,712 | A430S (GCG→TCG) | SP_1530 | MurE | |
| 2,125,225 | A586E (GCG→GAG) | SP_2205 | DHH subfamily 1 protein | |
| T4EP4 | 308,636 | Y586S (TAT→TCT) | SP_0336 | PBP2X |
| 324,228 | P80L (CCT→CTT) | SP_0350 | Cps4E | |
| 1,207,068 | Δ1 bp | SP_1274 | licD2 protein | |
| 1,280,284 | F218L (TTT→CTT) | SP_1358 | ABC transporter, ATP-binding/permease protein | |
| 1,442,712 | A430S (GCG→TCG) | SP_1530 | MurE | |
| 2,125,225 | A586E (GCG→GAG) | SP_2205 | DHH subfamily 1 protein | |
| T4EP5 | 136,365 | Δ1 bp | SP_0137 | ABC transporter ATP-binding protein |
| 308,635 | Y586H (TAT→CAT) | SP_0336 | PBP2X | |
| 945,790 | P509P (CCA→CCG) | SP_1003 | hypothetical protein | |
| 1,494,178 | V407V (GTT→GTC) | SP_1588 | pyridine nucleotide-disulfide oxidoreductase | |
| 2,048,117 | Δ1 bp | SP_2136 | choline-binding protein A | |
| 2,150,991 | L159L (CTA→TTA) | SP_2231 | copper ABC transporter permease | |
| T4EP6 | 308,636 | Y586C (TAT→TGT) | SP_0336 | PBP2X |
| 1,207,173 | G40E (GGA→GAA) | SP_1274 | licD2 protein | |
| T4EP7 | 103,388 | Δ1 bp | SP_0101 | MFS transporter |
| 308,458 | M527V (ATG→GTG) | SP_0336 | PBP2X | |
| 1,674,302 | +3 bp (+CCC) | SP_RS08765 | glycosyltransferase family 2 protein | |
| T4EP8 | 308,636 | Y586C (TAT→TGT) | SP_0336 | PBP2X |
| 1,207,823 | E257* (GAG→TAG) | SP_1274 | phosphorylcholine transferase LicD | |
| 1,635,159 | A507V (GCT→GTT) | SP_1732 | serine/threonine protein kinase pknB | |
| 1,674,302 | +1 bp (+C) | SP_RS08765 | glycosyltransferase family 2 protein |
Cps4E, capsular polysaccharide biosynthesis protein; MurE, UDP-N-acetylmuramoylalanyl-D-glutamate–2, 6-diaminopimelate ligase; PBP2X, penicillin-binding protein 2X.
a
Mutations listed were present in at least 50% of the sequenced population and were absent from either the parent strain or its drug-free passaged counterpart.