KEY RESOURCES TABL
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-BrdU antibody | Abcam | Cat# ab74545 RRID: AB_1523224 |
| Rabbit polyclonal to Ki67 | Abcam | Cat# Ab15580 RRID: AB_443209 |
| Anti-Mucin 2 | Santa Cruz Biotechnology | Cat# H-300 |
| Anti-GLP1 | Abcam | Cat# Ab22625 RRID: AB_447206 |
| Alexa Fluor 488 Goat Anti-Rabbit IgG | Invitrogen | Cat# A11008 RRID: AB_143165 |
| Alexa Fluor 555 Goat Anti-Rabbit IgG | Abcam | Cat# Ab150078 RRID: AB_2722519 |
| Anti-mouse CD16/CD32 | BioXCel | Cat# BE0307 |
| Anti-CD45 | Biolegend | Cat# 103155 RRID: AB_2650656 |
| Anti-CD11b | BD Biosciences | Cat# 557657 RRID: AB_396772 |
| Anti-Ly6G | BD Biosciences | Cat# 561236 RRID: AB_10611860 |
| Anti-Ly6C | Thermo fisher | Cat# 45-5932 RRID: AB_2723342 |
| Anti-CD11c | BD Biosciences | Cat# 563786 RRID: AB_2732919 |
| Anti-MHCII | BD Biosciences | Cat# 563415 RRID: AB_2738192 |
| Anti-CD64 | Biolegend | Cat# 139309 RRID: AB_2562694 |
| Anti-CD19 | Biolegend | Cat# 152404 RRID: AB_2629813 |
| Anti-IFN-γ | Biolegend | Cat# 505838 RRID: AB_2629667 |
| Anti-mouse IgA | eBioscience | Cat# 12-4204-82 |
| Anti-CD8α | Biolegend | Cat# 100725 RRID: AB_493425 |
| Anti-CD45 | Biolegend | Cat# 103155 RRID: AB_2650656 |
| Anti-CD4 | Thermo fisher | Cat# 47-0042-82 RRID: AB_1272183 |
| Anti-IL17A | BD Biosciences | Cat# 559502 RRID: AB_397256 |
| Anti-FoxP3 | Biolegend | Cat# 320014 RRID: AB_439750 |
| Anti-TCRβ | Biolegend | Cat# 109212 RRID: _313435 |
| Anti-CD4 | Biolegend | Cat# 100434 RRID: AB_893324 |
| Anti-mouse IgG antibody | Sigma-Aldrich | Cat# M2650-1ML RRID: AB_260486 |
| Anti-mouse IgG1 | SouthernBiotech | Cat# 1070-08 AB_2794413 |
| Anti-mouse IgG2C | SouthernBiotech | Cat# 1079-08 AB_2794467 |
| Normal Rat Serum | Thermo Scientific | Cat# 10710C RRID: AB_2532985 |
| Bacterial and virus strains | ||
| Bifidobacterium longum subsp. longum Reuter | ATCC | Cat# 15707 |
| Chemicals, peptides, and recombinant proteins | ||
| D- (+)-GLUCOSE | Sigma-Aldrich | Cat# G8270-1KG |
| Insulin | Sigma-Aldrich | Cat# 12643-25MG |
| Ampicillin | Sigma-Aldrich | Cat# A9518-100G |
| Neomycin | Sigma-Aldrich | Cat# N1876-100G |
| PEG 3350 | Sigma-Aldrich | Cat# P4338 |
| Tyloxapol | MedChemExpress | Cat # HY-B1068 |
| SytoBC | Invitrogen | Cat# S34855 |
| Lipopolysaccharides | Sigma-Aldrich | Cat# l2887-10MG |
| Phalloidin Tetramethyl rhodamine B isothiocyanate | Sigma-Aldrich | Cat# P1951-.1MG |
| DAPI Fluoromount-G® Mounting Medium | SouthernBiotech | Cat# 0100-20 |
| Stimulation cocktail | Thermo Scientific | Cat# 00-4975-93 |
| Brain heart infusion (BHI) medium | Sigma-Aldrich | Cat# 237500 |
| Paraformaldehyde | Thermo Scientific | Cat# J19943-K2 |
| Type IV collagenase | Sigma-Aldrich | Cat# C5138-5G |
| DNase I | Sigma-Aldrich | Cat# 10104159001 |
| Critical commercial assays | ||
| Triglycerides Reagent | Thermo Scientific | Cat# TR22421 |
| Cholesterol Reagent | Thermo Scientific | Cat# TR13421 |
| One-Step RT–PCR Kit with SYBR Green | Bio-Rad | Cat# 172-5151 |
| QIAamp DNA Stool Mini Kit | Qiagen | Cat # 51504 |
| QuantiFast SYBR Green PCR kit | Bio-Rad | Cat# 204054 |
| Lipocalin-2/NGAL ELISA | R&D Systems | Cat# DY1857 |
| FoxP3 staining buffer | Thermo Scientific | Cat# 00-5523-00 |
| Deposited data | ||
| 16S rRNA sequence | NCBI Sequence Read Archive | PRJNA873095 |
| Experimental models: Organisms/strains | ||
| Mice: C57BL/6 | The Jackson Laboratory | Cat # 000664 |
| Oligonucleotides | ||
| EUB338 probe: 5’-GCTGCCTCCCGTAGGAGT-3’, with a 5' labeling using Alexa 647 | Invitrogen | NA |
| 16S rRNA: 5′-AGAGTTTGATCCTGGCTCAG-3′ | Invitrogen | NA |
| 16S rRNA: 5′CTGCTGCCTCCCGTAGGAGT-3′ | Invitrogen | NA |
| 16S rRNA sequence primers of V4: 515FB 5′TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTGYCAGCMGCCGCGGTAA-3′ | Invitrogen | NA |
| 16S rRNA sequence primers of V4: 806RB 5′GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACNVGG | Invitrogen | NA |
| 16S rRNA sequence primers of V3-V4: 5′TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3′; | Invitrogen | NA |
| 16S rRNA sequence primers of V3-V4: 5′GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3′. | Invitrogen | NA |
| 18S rRNA: 5′GTAACCCGTTGAACCCCATT3′ | Invitrogen | NA |
| 18S rRNA: 5′CCATCCAATCGGTAGTAGCG3′ | Invitrogen | NA |
| IL-6: 5′GTGGCTAAGGACCAAGACCA3′ | Invitrogen | NA |
| IL-6: 5′GGTTTGCCGAGTAGACCTCA3′ | Invitrogen | NA |
| TNF-α: 5′CGAGTGACAAGCCTGTAGCC3′ | Invitrogen | NA |
| TNF-α: 5′CATGCCGTTGGCCAGGA3′ | Invitrogen | NA |
| MCP-1: 5′GCTGGAGCATCCACGTGTT3′ | Invitrogen | NA |
| MCP-1: 5′TGGGATCATCTTGCTGGTGAA3′ | Invitrogen | NA |
| Claudin-1: 5′ TCCTTGCTGAATCTGAACA3′ | Invitrogen | NA |
| Claudin-1: 5′AGCCATCCACATCTTCTG3′ | Invitrogen | NA |
| 36B4: 5′TCCAGGCTTTGGGCATCA3′ | Invitrogen | NA |
| 36B4:5′CTTTATTCAGCTGCACATCACTCAGA3′ | Invitrogen | NA |
| Software and algorithms | ||
| GraphPad Prism | GraphPad Software | NA |
| Image J | NIH | NA |