Figure 5.

Analyzing RNA quality using gel electrophoresis

1 μg of intact and degraded total RNAs extracted from HEK-293T cells were run on a 1.5% agarose gel. The 28S and 18S ribosomal RNA (rRNA) bands were detected by EtBr staining and the intensity ratio (28S rRNA/18S rRNA) was determined, which was 2.0 for the intact RNA sample. In the case of partially degraded sample, the rRNA ratio was 1.7, and for the completely degraded sample, we could not calculate the ratio.