Strand-specific RT second mixture
| Reagent | Amount |
|---|---|
| 5× SSIV buffer | 4 μL |
| 100 mM DTT | 1 μL |
| Recombinant RNase inhibitor | 1 μL |
| SuperScript IV Reverse Transcriptase (200 U/μL) | 1 μL |
For multiple samples, multiply each volume by the number of samples.
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