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. 2023 Jan 19;4(1):102007. doi: 10.1016/j.xpro.2022.102007

Strand-specific qPCR pre-mixture

Reagent Amount
2× SYBR reagent 7.5 μL
TDW 4.5 μL
3 μM qPCR primer mix 2 μL

For multiple samples, multiply each volume by the number of samples.