qPCR cycling conditions
| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 95°C | 3 min | 1 |
| Denaturation | 95°C | 5 s | 40 cycles |
| Annealing | 60°C | 10 s | |
| Melt Curve Stage 1 | 95°C | 15 s | 1 |
| Melt Curve Stage 2 | 60°C | 1 min | 1 |
| Melt Curve Stage 3 | 95°C | 1 s | 1 |
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