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. 2023 Jan 19;4(1):102007. doi: 10.1016/j.xpro.2022.102007

RNAscope single-molecule RNA-FISH wash buffer

Reagent Final concentration Amount
50× wash buffer 30 mL
ddH2O N/A 1,470 mL
Total 0.1% 1.5 L

Store at 20°C–25°C for up to one month. Prepare the solution in advance because the buffer contains detergent and easily forms bubbles. The solution can be poured down the sink after use.