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. 2001 Jul;69(7):4373–4381. doi: 10.1128/IAI.69.7.4373-4381.2001

FIG. 3.

FIG. 3

Electrophoretic analysis of PCR products derived from ialB mutant strain, SC1, and transcomplemented strain, SC2. PCR products were generated by amplification of genomic DNA from parent and recombinant strains using three amplimer sets (nptI [NPTI3′ and NPTI5′], ialB [IALBF and IALBR], and junction [jct] [NPTI5′ and IALBR]). Brackets below the gel indicate the amplimer set used in each reaction. Amplimer sets and template DNA for PCR used in this analysis are as follows. (A) Lane 1, NPTI3′ and NPTI5′, no template; lane 2, NPTI3′ and NPTI5′, JB584; lane 3, NPTI3′ and NPTI5′, SC1; lane 4, IALBF and IALBR, no template; lane 5, IALBF and IALBR, JB584; lane 6, IALBF and IALBR, SC1; lane 7, NPTI5′ and IALBR, no template; lane 8, NPTI5′ and IALBR, JB584; lane 9, NPTI5′ and IALBR, pSAC100; lane 10, NPTI5′ and IALBR, JB584 and pSAC100; lane 11, NPTI5′ and IALBR, SC1; lane 12, lambda DNA/HindIII and φX174 DNA/HaeIII markers. (B) Lane 1, IALBF, and IALBR, JB584; lane 2, IALBF and IALBR, SC1; lane 3, IALBF and IALBR, SC2; lane 4, NPTI5′ and IALBR, SC2; lane 5, lambda DNA/HindIII and φX174 DNA/HaeIII markers. PCR products were analyzed by ethidium bromide-stained agarose (1.2%, wt/vol) gel electrophoresis. Size standards in kilobase pairs are indicated on the right.