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. 2001 Jul;69(7):4373–4381. doi: 10.1128/IAI.69.7.4373-4381.2001

FIG. 5.

FIG. 5

Detection of the wild-type and mutated ialB genes in B. bacilliformis strains using DNA hybridization. (A) Ethidium bromide-stained agarose gel (1.2%, wt/vol) of ClaI-digested genomic DNA from the parental B. bacilliformis strain, JB584 (lane 2), the ialB mutant strain, SC1 (lane 3), and the transcomplemented strain, SC2 (lane 4). The shuttle plasmid used in transcomplementation, pSAC200, digested with ClaI is shown in lane 5, and DNA size standards (Lambda DNA/HindIII markers) are provided in lane 1. (B) Corresponding Southern blot hybridized with the ialB probe. Lane 1, DNA size standards; lane 2, single hybridization band of ∼23 kbp from the parental B. bacilliformis strain, JB584; lane 3, two-band hybridization pattern from the disrupted ialB gene in the ialB mutant strain, SC1; lane 4, two-band hybridization pattern from the disrupted ialB gene, as well as the ∼5.4-kbp hybridization band from pSAC200 in the transcomplemented strain, SC2; lane 5, single hybridization band from pSAC200. Size standards in kilobase pairs are indicated on the left.