TABLE 1.
Bacteria and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| B. bacilliformis | ||
| JB584 | Transformation-competent strain of B. bacilliformis | 5 |
| SC1 | JB584 with ialB interrupted by pSAC100 (Kmr, ialB mutant) | This study |
| SC2 | SC1 complemented in trans with pSAC200 (Kmr Cmr, ialB+) | This study |
| E. coli | ||
| DH5α | Host strain for cloning and plasmid propagation | Gibco-BRL |
| M15 | Host strain for fusion protein expression | 41 |
| Plasmids | ||
| pIAL1 | pUC19 containing ialA and ialB of B. bacilliformis | 27 |
| pIALB | pUC19 containing ialB of B. bacilliformis | 27 |
| pQE-31 | Expression vector | Qiagen Inc. |
| pREP4 | Plasmid encoding lacI | Qiagen Inc. |
| pQIALB | pQE-31 with 574-bp PvuII-PstI fragment encoding ialB minus its secretory signal sequence plus 15 nucleotides | This study |
| pUB1 | B. bacilliformis suicide plasmid; Kmr | 5 |
| pSAC100 | pUB1 with an internal 430-bp PvuII-MfeI fragment of ialB; Kmr | This study |
| pBBR1MCS | B. bacilliformis shuttle vector; Cmr | 18 |
| pSAC200 | Complementation plasmid; pBBR1MCS with 756-bp SwaI-BamHI fragment containing intact ialB; Cmr | This study |