Table 2. Kinetic Parameters of the Interaction between Mut FcγRIIIa and Expi293-Derived Antibody Ligands at 25 °Ca.
| kon (×104 M–1 s–1) | koff (×10–2 s–1) | KD (×10–6 M) | |
|---|---|---|---|
| rituximabb | 3.96 ± 0.11 | 5.44 ± 0.11 | 1.38 ± 0.04 |
| trastuzumabb | 3.00 ± 0.10 | 5.83 ± 0.16 | 1.95 ± 0.05 |
| adalimumab | 4.00 ± 0.19 | 6.20 ± 0.37 | 1.55 ± 0.05 |
| IgG1-Fc | 4.78 ± 0.10 | 8.02 ± 0.17 | 1.68 ± 0.07 |
| MBP-Fc | 1.84 ± 0.05 | 6.35 ± 0.08 | 3.47 ± 0.10 |
a
At least five independent measurements were carried out. The average values with standard errors are shown.
b
Data from our previous study.19