Figure 3.

Extinction of CTA enhances, whereas reinstatement decreases, the excitability of LIV–VI aIC-BLA projecting neurons. A, Experimental design of behavioral procedures conducted to compare the intrinsic properties of LIV–VI aIC-BLA neurons following CTA Extinction (n = 5, animals, 14 cells) and Reinstatement (n = 3 animals, 15 cells). B, The graph showing the reduced aversion following the successful extinction in both treatment groups. C, Data showing the saccharin consumption on the test day following successful extinction and Reinstatement of CTA. CTA reinstated mice showed significantly reduced saccharin consumption compared with extinguished mice. p = 0.0179, Mann–Whitney test. D, Representative traces of LIV–VI aIC-BLA projection neurons firing from the two treatment groups. Scale bars: 20 mV and 50 ms horizontal from 300-pA step. E, Excitability in LIV–VI aIC-BLA neurons was significantly different among the treatment groups. Two-way repeated measures ANOVA, Current × Treatment: p < 0.0001. F, Action potential threshold in the Reinstatement group (−29.43 ± 1.731 mV) was enhanced compared with Extinction (−36.06 ± 1.481 mV). p = 0.0076, Unpaired t test. G, The membrane time constant following Reinstatement (25.48 ± 1.58 ms) was significantly enhanced compared with Extinction (17.55 ± 2.684 ms, p = 0.047). p = 0.0153, Unpaired t test. For panels D–F: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data are shown as mean ± SEM. Extinction of CTA enhances the excitability of burst spiking LIV–VI aIC-BLA projecting neurons compared with CTA reinstatement (see Extended Data Fig. 3-1).