Fig. 1.

Acute deletion of TRPML1 inhibits osteoclastogenesis. (A, B) BMMs in primary culture were incubated with 50 ng/mL RANKL for the indicated times and the levels of TRPML1 mRNA (A) and protein (B) were analyzed. (C) BMMs were treated with shTRPML1, and shGFP as a negative control, for 72 hours, and the level of TRPML1 mRNA (left blots) and protein (right blots) were determined. (D) Osteoclastogenesis was induced by treating BMMs that were treated with shGFP or shTRPML1 with 50 ng/mL RANKL for 3 days. Shown are representative images (Scale bar = 100 μm) from 3 experiments. Depletion of TRPML1 suppressed osteoclastogenesis as revealed by reduced TRAP staining and multinucleated cells. *p < 0.05.