Fig. 3.

Aberrant NFATc1 activation and RANKL-mediated osteoclastogenesis in TRPML1^−/− mice. BMMs isolated from WT and TRPML1^−/− mice were cultured and used for the experiments below. (A) RANKL-mediated NFATc1 expression is suppressed in TRPML1^−/− BMMs. β-actin was used as a loading control. (B) Deletion of TRPML1 reduces RANKL-mediated MNC formation. The number of TRAP-positive MNCs, which are identified by the presence of more than 3 nuclei and cell size larger than 100 μm in diameter, present in each well were counted. The columns are the mean±SD of 5 experiments. (Scale bar = 100 μm). (C) Deletion of TRPML1 alters expression of differentiation marker genes for RANKL-mediated osteoclastogenesis. Results are presented as fold increase compared to WT cells treated with DW. *p < 0.05, **p < 0.01.