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. Author manuscript; available in PMC: 2023 Jan 19.
Published in final edited form as: J Invest Dermatol. 2019 Aug 28;140(3):676–687.e6. doi: 10.1016/j.jid.2019.08.435

Fig. 2. Antibiotics affect microbial diversity in the gut and not in the skin.

Fig. 2.

(a) Isolates from total skin homogenates at 35 weeks were pre-enriched for microbial DNA before completing 16S rRNA analysis. Microbial diversity is maintained in the skin after antibiotic treatment. (b) Relative bacterial DNA content relative to reference strain R. productus determined by qPCR in samples from 3 control, 5 ampicillin-treated or 9 neomycin treated mice. The amounts significantly differed between ampicillin and neomycin groups, with concentrations trending towards a decrease and increase compared to controls, respectively. (c) Fecal DNA analysis reveals marked skewing of the microbiome. Ampicillin treatment is associated with enrichment of Pseudomonas species in the gut, whereas neomycin treatment enriched for the Bacterioides genus. (*Genus not identifiable).