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. Author manuscript; available in PMC: 2023 Jan 19.
Published in final edited form as: J Invest Dermatol. 2019 Aug 28;140(3):676–687.e6. doi: 10.1016/j.jid.2019.08.435

Fig. 6. Antibiotic treatment skews T-cell distribution and functionality.

Fig. 6.

(a) Example FACS plots and (b) bar graphs of total T cells, CD4+ and CD8+ T cells among CD45+ splenocytes. (c) IFN-γ and IL-17A were measured in CD8+ T cells from antibiotic-treated or control mice after 5 hrs incubation with or without leukocyte activation cocktail (LAC). (d) IFN-γ and IL-17A+ cells in live CD8+ T cells among LAC or media-treated controls cells. (e) IFN-γ and (f) IL-17 were measured by ELISA in splenocyte supernatants from antibiotic-treated and control mice stimulated with media, Tyrosinase369–377 (30μg/ml) or CD3/CD28 activator beads for 48 hrs. (*p<0.05, **p<0.01).