Figure 6.

UXT facilitates the interaction of SQSTM1 and STING1. (A, B, C and D) HEK293T cells were transfected with the indicated plasmids. Then, cell lysates were immunoprecipitated with an anti-Flag antibody and then immunoblotted with the indicated antibodies. (E) MEFs were treated with chloroquine and then stimulated with HTDNA for 3 h, and the cell lysates were immunoprecipitated with an anti-UXT antibody or normal IgG, and then immunoblotted with the indicated antibodies. (F) MEFs were treated with chloroquine and then stimulated with cGAMP for 3 h, and the cell lysates were immunoprecipitated with an anti-UXT antibody or normal IgG, and then immunoblotted with the indicated antibodies. (G) MEFs were treated with chloroquine and then stimulated with HTDNA for 3 h, and the cell lysates were immunoprecipitated with an anti-UXT antibody or normal IgG, and then immunoblotted with the indicated antibodies. (H) MEFs transfected negative control (NC) or Uxt siRNAs were treated with chloroquine and then stimulated with HTDNA (2 μg per well) for 3 h, and the cell lysates were immunoprecipitated with an anti-SQSTM1 antibody or normal IgG, and then immunoblotted with the indicated antibodies. (I) MEFs transfected negative control (NC) or Uxt siRNAs were treated with chloroquine and then stimulated with cGAMP (2 μg per well) for 3 h, and the cell lysates were immunoprecipitated with an anti-SQSTM1 antibody or normal IgG, and then immunoblotted with the indicated antibodies. (J) MEFs transfected control vectors (Vec) or UXT expression plasmids (UXT) were treated with chloroquine and then stimulated with cGAMP (2 μg per well) for 3 h, and the cell lysates were immunoprecipitated with an anti-SQSTM1 antibody or normal IgG, and then immunoblotted with the indicated antibodies.