Figure 7.
LAMP1 is enriched on SQSTM1-positive puncta as compared with SQSTM1-positive aggresomes in astrocytes. (A-H) Immunostain analysis of primary astrocytes (5–7 DIV) treated with DMSO, 0.5 µM MG132 or 10 nM epoxomicin for 24 h; 100 nM Baf A1 (or equivalent volume of DMSO as a solvent control) was included in the last 4 h. Maximum projections of z-stacks of GFP-LC3 transgenic astrocytes immunostained for GFP, SQSTM1, and LAMP1. Corresponding line scans are drawn from the cell periphery toward the perinuclear region (left to right); asterisks indicate puncta or aggresomes with overlapping markers. Dashed line in maximum projection denotes location of line scan. Bar: 10 µm.