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. 2022 Jul 27;19(2):644–659. doi: 10.1080/15548627.2022.2094671

Figure 1.

Figure 1.

Epg5 knockout female mice are subfertile. (a) Immunoblotting of ovary samples for the EPG5 protein and ACTB (internal control). (b) Images of ovaries from control and epg5 knockout mice at 8 weeks. Scale bars: 2 mm. (c) The ovary-to-body weight ratio of the control and epg5 knockout mice. (d) Subfertility of female epg5 knockout mice. Both control (n = 5) and epg5 knockout (n = 5) females were mated with WT male mice; continuous breeding showed the cumulative number of progeny per female mouse. The asterisks indicate statistically significant differences. (e) the average litter size of control (n = 5) and epg5 knockout (n = 5) females. (f) Representative histology of ovaries sections from 16-week-old epg5 knockout females stained with hematoxylin and eosin. (g) Numbers of follicles at different stages in ovary sections from 16-week-old control and epg5 knockout mice (n = 4 for each group). Follicles were counted; black arrowheads in F point to follicles at different stages. PmF, primordial follicle; PF, primary follicle; SF, secondary follicle; AF, antral follicle. CL: Corpus luteum. Scale bars: 100 µm. Data are expressed as the mean ± S.E.M. *P < 0.05, **P < 0.01. ***P < 0.001.