Figure 2.

Oocytes from epg5 knockout mice are able to complete meiotic maturation. (a) The average number of ovulated eggs were evaluated in control and epg5 knockout mice (n = 7 for each group). (b) Quantitative analysis of first polar body (Pb1) extrusion rate in epg5 knockout and control (n = 234) oocytes. (c) Representative images of oocytes from WT and epg5 deficiency mice after 14 h culture. scale bars: 100 μm. Immature GV oocytes isolated from 3-week-old control and epg5 knockout mice were cultured in vitro to check the maturational progression. (d) Quantitative analysis of mii oocytes of control and epg5 knockout mice after 14 h culture. (e) Confocal microscopy results showing spindle assembly collected from cultured MII oocytes of control and epg5 knockout mice. (f) Quantitative analysis of abnormal MII with a deformed spindle and misaligned chromosomes. Scale bar: 20 μm. Data are expressed as mean ± S.E.M. n.s., nonsignificant.