Fig. 6.

(A) Reaction rates that approximate the changes in metabolite abundances induced by 30 minutes of compression as computed by ASCA, r₃. These rates are consistent with increased synthesis of aspartate and glutamine, all needed for protein synthesis as required by chondrocyte homeostasis. (B) Reaction rates ${\mathbf{r}}_{\mathbf{1}}^{\mathbf{i}}$ for each donor i, for the periods of 0–30 minutes of compression, calculated from the differences in the medians of the samples for each time point. These are consistent with the compression rates, demonstrating that the overall shift in metabolism within donors comes as a result of compression over time. Reaction labels defined in Supplement S1.