Table 1. Total and lysine methylated peptides identified in wild type and SETD2-KO human kidney cell lines.
WT = wild type. KO = knock out. H = heavy. L = light. A-D refer to biological replicates. meK = lysine methylation. me1K = lysine monomethylation. me2K = lysine dimethylation. me3K = lysine trimethylation. IP = immunoprecipitation.
| Replicate | A | B | C | D |
|---|---|---|---|---|
|
| ||||
| WT | H | L | L | H |
| KO | L | H | H | L |
|
| ||||
| Total Protein | ||||
|
| ||||
| Total Proteins Quantified | 4863 | 5190 | 5002 | 5283 |
| Total Peptides Quantified | 27069 | 31773 | 29817 | 29800 |
|
| ||||
| meK IP | ||||
| meK Peptides Quantified | ||||
| me1k | 37 | 33 | 29 | 39 |
| me2k | 14 | 14 | 11 | 9 |
| me3k | 12 | 10 | 11 | 10 |
|
| ||||
| meK Proteins Quantified | ||||
| me1k | 29 | 27 | 24 | 31 |
| me2k | 8 | 10 | 8 | 6 |
| me3k | 9 | 7 | 8 | 8 |