Table 2. Peptides demonstrating decreased lysine methylation in SETD2-KO human kidney cell lines.
“Position” corresponds to location of methylated lysine residue within the full-length protein. “MeK” indicates whether the lysine residue was mono-, di-, or tri-methylated. “Methyl K Probabilities” indicates the relative probability that the indicated lysine residue was modified. A-D indicates the four biological mass spectrometry replicates and the ratio of the wild type (WT) and SETD2-knock out (KO) ratios (i.e., WT/KO ratio). If the lysine predicted to be modified corresponds to a canonical SETD2 methylation motif (i.e., KxP or KxxG), it was indicated with “x”. Only peptides with a single methylated lysine whose WT/KO ratio were >1 in all replicates and whose average was >1.25 are listed. See Sup Table 1 for the complete results.
| Protein | UniProt ID | Position | MeK | Methyl (K) Probabilities | A | B | C | D | K Motif | KxP | KxxG |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||||
| AHNK_HUMAN | Q09666 | 1208 | 1 | ISMPDVDLHLK(0.403)GPK(0.597) | 2.15 | KVKG | X | ||||
| AHNK_HUMAN | Q09666 | 2194 | 1 | ISMPDVNLNLK(0.854)GPK(0.146) | 2.16 | KGPK | X | ||||
| ANXA2_HUMAN | P07355 | 104 | 1 | SALSGHLETVILGLLK(1) | 1.58 | 1.83 | 1.17 | 1.35 | KTPA | X | |
| BCAR3_HUMAN | O75815 | 334 | 1 | ALSLK(1)AHQSESYLPIGCK | 1.91 | KAHQ | |||||
| EF1A1_HUMAN | P68104 | 55 | 1 | GSFK(1)YAWVLDK | 1.13 | 1.67 | 1.10 | KYAW | |||
| EF1A1_HUMAN | P68104 | 79 | 1 | GITIDISLWK(0.986)FETSK(0.014) | 1.47 | KFET | |||||
| EF1A1_HUMAN | P68104 | 79 | 2 | GITIDISLWK(1)FETSK | 1.47 | KFET | |||||
| EF1A1_HUMAN | P68104 | 84 | 1 | GITIDISLWK(0.5)FETSK(0.5)YYVTIIDAPGHR | 1.47 | KYYV | |||||
| EF1A1_HUMAN | P68104 | 84 | 2 | GITIDISLWK(0.5)FETSK(0.5)YYVTIIDAPGHR | 1.47 | KYYV | |||||
| EF1A1_HUMAN | P68104 | 165 | 3 | MDSTEPPYSQK(1)R | 1.23 | 1.50 | 2.20 | 1.42 | KRYE | ||
| EF1A1_HUMAN | P68104 | 318 | 1 | NVSVK(1)DVR | 1.26 | 3.87 | 1.96 | 1.09 | KDVR | ||
| EF1A1_HUMAN | P68104 | 318 | 2 | NVSVK(1)DVR | 1.30 | 2.83 | 1.69 | 1.04 | KDVR | ||
| GCN1_HUMAN | Q92616 | 1663 | 1 | DLAPYLPSVTPGLK(1)ASLLDPVPEVR | 1.30 | 1.87 | 1.65 | KASL | |||
| GRP78_HUMAN | P11021 | 585 | 3 | LGGK(1)LSSEDKETMEK | 1.48 | 1.40 | 1.43 | 1.05 | KLSS | ||
| H7BZC6_HUMAN | H7BZC6 | 58 | 1 | LK(1)GAILTTMLATR | 1.64 | KGAI | |||||
| HS90A_HUMAN | P07900 | 615 | 1 | IMK(1)AQALR | 1.24 | 1.50 | 1.57 | 1.45 | KAQA | ||
| HS90B_HUMAN | P08238 | 607 | 1 | IMK(1)AQALR | 1.24 | 1.50 | 1.57 | 1.45 | KAQA | ||
| K7EK07_HUMAN | K7EK07 | 37 | 2 | SAPSTGGVK(0.977)K(0.023)PHR | 1.23 | 1.55 | KKPH | X | |||
| K7EK07_HUMAN | K7EK07 | 38 | 2 | SAPSTGGVK(0.5)K(0.5)PHR | 1.55 | KKPH;KPHR | X | ||||
| RTF1_HUMAN | Q92541 | 587 | 1 | ALVAESHNMK(1)NQQMDPFTR | 1.14 | 1.67 | 1.54 | KNQQ | |||