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. 2022 Dec 6;11(23):e027538. doi: 10.1161/JAHA.122.027538

Figure 5. The increased endothelium‐dependent relaxation with lowering superoxide level in HFHSD‐fed EE2KO mice.

Figure 5

Vascular relaxation of aortic rings with acetylcholine (A) and sodium nitroprusside (B) (Control‐HFHSD, n=10; EE2KO‐HFHSD, n=7). Dihydroethidium staining of aortic rings (C) and quantification of dihydroethidium fluorescence intensity (D) in Control‐HFHSD and EE2KO‐HFHSD mice (Control‐HFHSD, n=6; EE2KO‐HFHSD, n=3). Scale bar, 50 μm. Error bars represent SEM. Vascular relaxation was analyzed by 2‐way ANOVA with repeated measures followed by the post hoc Bonferroni correction for multiple comparisons (A and B). Between‐group difference was compared by Student t test (D). *P<0.05, **P<0.01 (compared with Control‐HFHSD mice). a.u. indicates arbitrary unit; EE2KO, endothelial‐specific extracellular signal‐regulated kinase 2 knockout; and HFHSD, high‐fat/high‐sucrose diet.