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. 2022 Dec 6;11(23):e027538. doi: 10.1161/JAHA.122.027538

Figure 11. Phosphorylation of AKT and eNOS is increased in HUVECs treated with lentivirus for ERK2 suppression and incubated with palmitic acid.

Figure 11

A, Western blotting of HUVECs treated with lentivirus for ERK2 suppression and incubated with palmitic acid. B, Relative levels of AKT phosphorylation. C, Relative levels of eNOS phosphorylation. D, Relative levels of ERK2 expression (n=5 per group). Error bars represent SEM. Between‐group differences were compared by Student t test (B through D). *P<0.05, **P<0.01 (compared with sh Control). AKT indicates protein kinase B; eNOS, endothelial nitric oxide synthase; ERK2, extracellular signal‐regulated kinase 2; HUVECs, human umbilical vein endothelial cells; p‐AKT, phosphorylation of AKT; p‐eNOS, phosphorylation of eNOS; p‐ERK2, phosphorylation of ERK2; t‐AKT, total AKT; t‐eNOS, total eNOS; t‐ERK2, total ERK2; and sh, small hairpin RNA.