Figure 6.

Integrated pan-genome analysis comparing SNP differences between pairs of nontuberculous mycobacteria isolates within clusters (x-axis) versus percentage shared accessory genome (y-axis). Mycobacterium abscessus (ssp.) abscessus clusters are represented by circles, Mycobacterium avium clusters are represented by triangles, the Mycobacterium intracellulare cluster is represented by a diamond, and the Mycobacterium abscessus ssp. massiliense cluster is represented by an upside-down triangle. Clusters A (blue circles) and J (scarlet circles), as well as M. avium clusters D (caramel triangles) and I (chartreuse triangles), demonstrated episodes of healthcare-associated opportunities for transmission. Cutoff points of ⩽10 SNPs and ⩾95% shared accessory genome are noted in the dashed line box, representing the most stringent degree of clustered isolate similarity. M. avium cluster D, with sequential healthcare-associated opportunity for transmission from D1 to D2 and D2 to D3, M. abscessus cluster K with no evidence of healthcare-associated opportunity for transmission, and M. abscessus cluster E, with no evidence of healthcare-associated opportunity for transmission but residing in the same watershed, fall within the stringent genetic similarity cutoffs.