Fig 3. gPhage library construction and phage selection.

DNA inserts (fragments of T. cruzi genomic DNA) were cloned into the gPhage vector (top). Because inserts were randomly inserted into the vector into any of all possible reading frames, only a fraction of phage particles displays a T. cruzi-derived peptide (middle). However, with the successive rounds of biopanning, only phage displaying a T. cruzi-derived peptide are selected and enriched while the remaining phage particles are removed with wash (bottom).