Table 2. Antigens and epitopes preferentially recognized by patients’ IgG at the beginning of treatment (T0).

Patient	Antigen	Accession	Frequency (%)	Consensus sequence
1	<17.2% in-frame inserts
2	Putative surface antigen 2 (CA-2) Trans-sialidase, putative, partial Trans-sialidase, putative, partial Putative trans-sialidase Trans-sialidase, putative	PWU84979.1 EKF99511.1 EKF98285.1 PWU90932.1 EKG00226.1	95.69 3.73 0.40 0.13 0.05	GQAAAGDKPSPFGQAAAGDKPPPFGQAAAGDKPSPFGQAAAGDK KASVHVDGESLGNEEVPLTGEKPPE TSLGEEEVPLTGEAPLGLV PDSFSSTNVSGGVDAAPAPSSTASG APSSTASGETKIPSELNATVPSDHDILLEFRELA
3	Putative surface antigen 2 (CA-2) Putative surface antigen 2 (CA-2)	PWU84979.1 PWU84979.1	99.69 0.31	QAAAGDKPPPFGQAAAGDKPSPFEQAAAG GDRPSPFEQAAAGAKPSPFGQ
4	Putative surface antigen 2 (CA-2) Mucin TcMUCII Mucin TcMUCII, putative Hypothetical protein TCSYLVIO_010170 Monoglyceride lipase, putative, partial Hypothetical protein TCSYLVIO_009815 Hypothetical protein MOQ_008444, partial Putative 60S ribosomal protein Mucin TcMUCII, putative Hypothetical protein	PWU84979.1 XP_808990.1 EKF29049.1 EKF98926.1 EKF27476.1 EKF99266.1 EKF27823.1 PWU84687.1 EKF29049.1 XP_822001.1	40.49 23.93 9.82 9.82 4.91 3.68 2.45 1.84 1.84 1.23	AAGDKPPPFGQAAAGDKPSPFGQAAAG PSTTTTEAPTTTTTRAPSRLREID NTARNTEAPITTTTTRAPSRFREID HDIYHQRNPNSSGSVGRRGGVWGRTVSPASTEQGL VPLTARYGAEMMRAIDD ETVDALNEKVWTAEFRQIDTE TDTTPLVIVGPETSVAPVAAQRAIDTV VTGTAMPRGMQAFPLRDSAETI AGNTEAPATATTRAPSRLREID RQRLVDPSEPPTSPASTEMDETGKAST
5	Putative surface antigen 2 (CA-2) Putative microtubule-associated protein Hypothetical protein C4B63_133g26 Microtubule-associated protein, partial Hypothetical protein C4B63_76g64 Hypothetical protein C4B63_76g64 Surface antigen 2 (CA-2) Hypothetical protein Putative surface antigen 2 (CA-2) Putative microtubule-associated protein	PWU84979.1 PWU97874.1 PWU86111.1 XP_803031.1 PWU88332.1 PWU88332.1 XP_818927.1 XP_813515.1 PWU84979.1 PWU97874.1	70.41 24.05 4.87 0.32 0.20 0.06 0.04 0.02 0.02 0.02	GQAAAGDKPSPFGQAAAGDKPSPFGQAAAGDKPSPFGQAAAGDK VDPSAYKRALPQEEEEDVGPRHVDPDHFRSTTQ PFKSVFGAPSSTDAKPPAESPFKS MGPSAQNYDTQEEEDVGPRHVDPDHFRST ATHERAVEALAAEEDAARGQLVGGE AAVDELGEAFRSATHERAVEALAAEEDA SPFGQAAAGDKPPLFGQGTVFDAS DAKPPAESPFKGGFGAPSSTVAKPPGESPFKN AAGDKPPPVEQAAGGDRPSPFEQA YKRALPEEGQGDLGPRQVDPDHFRSTTQDA
6	putative surface antigen 2 (CA-2) hypothetical protein C4B63_9g493 putative mucin TcMUCII putative microtubule-associated protein hypothetical protein C4B63_9g493 mucin TcMUCII, putative	PWU84979.1 PWU99324.1 PWU85205.1 PWU97874.1 PWU99324.1 EKF29049.1	76.33 21.11 2.17 0.28 0.06 0.04	SPFGQAAAGDKPPPFGQAAAGDKPSPFGQAAAGDKPPL SAPAAGGFGSATTTSAPAVGGFGSATT TPSTTTTGTPTTTTTRAPSRLREIDS LPQEEQEDVGPRHVDPDHFRSTTQDAY VGGFGSAAHTSTPGVGGFGSATTT PINTAGKTEAPTTTTITHAPSRLREID
7	Too few peptide sequences
8	Putative surface antigen 2 (CA-2)	PWU84979.1	100.00	DKPPPFGQAAAGDKPSPFGQAAAGDKPSPF
9	<17.2% in-frame inserts
10	Mucin TcMUCII Mucin TcMUCII Mucin TcMUCII, putative Mucin TcMUCII, putative, partial	XP_821913.1 XP_820005.1 EKG05373.1 EKG03739.1	95.90 2.97 0.85 0.28	TEASTTTTTRAPSRLREID TTTTTTEAPNTTIPRAPSRLREID TTTTTTADPTTTSARTPSRLREID TTTTTTSAPEAPSNTTMNTEAPTPTTSRAPLRLREIDV
11	Putative microtubule-associated protein Putative surface antigen 2 (CA-2) Flagellar attachment zone protein 1 Putative microtubule-associated protein	PWU97874.1 PWU84979.1 PWU95940.1 PWU97874.1	93.43 6.45 0.10 0.01	ALPQEEEEDVGPRHVDPDHFRSTTQ QAAAGDKPPPFGQAAAGDKPSPFGQAAAGDKPS EELEQKAAENERLAEELEQKAAENE EEQEDVGPRHVGPDQFPPTTQDAYRPVDPS
12	Putative surface antigen 2 (CA-2) Putative trans-sialidase Putative microtubule-associated protein Putative surface antigen 2 (CA-2)	PWU84979.1 PWU87189.1 PWU97874.1 PWU84979.1	71.29 28.31 0.24 0.16	AAGDKPSPFGQAAAGDKPSPFGQAAAGDKPLPFEQA MPAGTSEEGSRDDSPMPAGASEEGSRDD SAYKRALPQEEEEDVGPRHVDPDHFRST AAGEKLPFGKAAAGDKPPPFGQA
13	Putative surface antigen 2 (CA-2) Putative microtubule-associated protein Hypothetical protein Hypothetical protein	PWU84979.1 PWU97874.1 XP_820768.1 XP_810212.1	96.10 1.74 1.67 0.49	SPFGQAAAGDKPSPFGQAAAGDKPSPFGQA LPQEEQEDVGPRHVDPDHFRSTTQ DSVTLTSLWSSRTAQARPASMRLVDGV PAVGGFGSATTTSAPAAGGFGSATTTSAPA
14	Putative surface antigen 2 (CA-2) Putative surface antigen 2 (CA-2)	PWU84979.1 PWU84979.1	96.84 3.16	GQAAAGDKPPPFGQAAAGDKPSPFGQ DKPSPFGQVAAGEKPPPFGQAAAGEK
15	<17.2% in-frame inserts
16	Putative surface antigen 2 (CA-2) Retrotransposon hot spot (RHS) protein	PWU84979.1 EKF99800.1	99.95 0.05	GQAAAGDKPPPFGQAAAGDKPSPFGQA MNCTPCGPFCWGMAGGYVGWNYCLRQHGRRLTFM
17	Putative surface antigen 2 (CA-2) Putative microtubule-associated protein Putative microtubule-associated protein Putative microtubule-associated protein Putative microtubule-associated protein	PWU84979.1 PWU97874.1 PWU97874.1 PWU97874.1 PWU97874.1	97.31 2.66 0.01 0.01 0.01	AAGDKPPPFGQAAAGDKPPPFGQAAAGDKP ALPQEEEEDVGPRHVDPDHFRSTTQD SAYKRALPQEEEGGVGPGPVDPAHFRSP SAYKRALPQEEEEDVGPGQVDPDQFRWT SAYKRALPQEKEGDGGPGHVVPDHFRST
18	<17.2% in-frame inserts
19	<17.2% in-frame inserts
20	<17.2% in-frame inserts