FIG. 2.
Amino acid alignment of the active site region of A. pyogenes NanH, A. viscosus NanH (61), M. viridifaciens NedA (45), and B. fragilis NanH (1). Amino acids identical to the NanH sequence are boxed in black. Conservative substitutions are boxed in gray. The RIP/RLP motif and the two additional catalytic arginine residues are indicated by the arrows. The Ser-X-Asp-X-Gly-X-Thr-Trp (Asp box) motifs are underlined. Amino acid numbers for each protein are indicated on the right.