FIG. 3.

Overexpression and purification of His-NanH. Whole-cell lysates of IPTG-induced cultures of DH5αMCR(pTrcHis B) (lanes 1) and DH5αMCR(pJGS306) (lanes 2) and 100 ng of purified His-NanH (lanes 3) were subjected to SDS–10% PAGE. Separated proteins were stained with Coomassie brilliant blue (A) or were transferred to nitrocellulose by Western blotting and immunostained with 1/100 E. coli-adsorbed preimmune serum (B) or 1/100 E. coli-adsorbed His-NanH antiserum (C). The positions of the molecular size standards are shown (in kilodaltons) on the left. The arrow indicates the position of His-NanH.