FIG. 4.

Scheme for insertional inactivation of the A. pyogenes nanH gene. A ClaI fragment containing nanH was replaced with the erm(X) gene of pNG2, to construct pJGS357. This plasmid was introduced into A. pyogenes strain BBR1 by electroporation. Reciprocal recombination, indicated by the dashed lines, resulted in replacement of the endogenous nanH gene in the BBR1 chromosome with the erm(X) gene to construct NANH-1. B, BamHI; C, ClaI; E, EcoRI; H3, HindIII. Only the insert portion of pJGS357 is to scale.