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FPLC gel filtration chromatography of lutein ND. A 400 μl aliquot of freshly prepared lutein ND (corresponding to 0.8 mg apoA-I and 0.4 mg lutein) was analyzed by FPLC gel filtration chromatography, with absorbance continuously monitored at 280 nm (A). Fractions corresponding to Peak 1 from Panel a were pooled, concentrated and subjected to FPLC gel filtration analysis under the same conditions (B).
