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. 2022 Dec 19;30(1):91–98. doi: 10.1038/s41594-022-00891-8

Fig. 4. Nog2 does not engage with unmodified G2922.

Fig. 4

a, Cryo-EM structure of pre-60S in complex with Nog2 bound to H92 from methyltransferase-defective spb1-D52A mutant cells in which G2922 is unmodified (crown view, left). The unrotated 5S rRNA is indicated, along with Nog2, H92, Rsa4 and Bud20. b,c, Atomic models of unmodified G2922 and Nog2 (b) and modified G2922 and Nog2 (accession no. PDB 3JCT) (c). d, Cartoon model for Nog2 gating of 60S assembly and nuclear export, dependent on methylation of G2922. NPC, nuclear pore complex.