Extended Data Fig. 2. Details of interactions between Nog2 and H92/Gm2922.
A. Modeling of active site of Nog2 with Gm2922 (accession no. PDB 3JCT) shown and Switch I (G2) and Switch II (G3) colored. B. Cryo- EM map of Nog2 (EMDB-6615) showing clear density for 2′-O-methyl group at G2922. C. Two-dimensional map of interactions between Nog2 amino acids and RNA elements of H92. Nucleotides and amino acids are depicted as rectangles, relevant phosphates are shown as orange circles. Hydrogen bonds between bases are shown as dashed lines, and the wobble base pair between A2919 and C2927 is shown as a dot. Interactions between amino acids and H92 are depicted as dotted lines. Threonine 195 (T195) was selected as a candidate for a suppressing amino acid substitution (Fig. 3). D. Western blots to verify expression of WT NOG2-3xFLAG and variants S208A, R389S and double mutant. Glucose-6-phosphate dehydrogenase (G6PD) was used as a loading control.