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. 2022 Dec 19;30(1):91–98. doi: 10.1038/s41594-022-00891-8

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Extended Data Fig. 3 — A. RNase T1 cleaves 3′ to guanosines in single-stranded RNA. However, 2′-O-methylated guanosine is resistant to cleavage. Guanosines base-paired with the reverse transcription primer are also protected. After cleavage with RNase T1, reverse transcription will generate a 30 nt product if G2922 is sensitive to cleavage (that is not methylated), or a 34 nt product if G2922 is protected from cleavage (that is methylated).