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. 2022 Dec 19;30(1):91–98. doi: 10.1038/s41594-022-00891-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — A. Immunopurifications from the indicated strains, stained with Coomassie blue. Proteins identified by mass spectrometry are indicated. B. Volcano plot showing log₂ fold change of factors that co- immunopurify with Nog2-3xFLAG, in unmodified G2922 (spb1-D52A) versus modified Gm2922 (WT SPB1). Dashed lines intersecting x-axis indicate a log₂ fold change of 1, and intersecting y- axis indicate a -log₁₀ (p) value of 1.3 (p = 0.05).

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