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. 2022 Dec 19;30(1):91–98. doi: 10.1038/s41594-022-00891-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — A. Raw micrograph of Nog2-3xFLAG particles immunopurified from the pGAL:SPB1 strain AJY4425 expressing spb1- D52A. Representative of 25,956 micrographs. Scale bar = 100 nm. B. Cryo-EM data processing scheme by which the Nog2-3xFLAG structure was obtained.