Table 1.
Cryo-EM data collection, refinement and validation statistics
| Nog2-3×FLAG unmodified G2922 (EMD-26485; PDB 7UG6) | |
|---|---|
| Data collection and processing | |
| Magnification | ×29,000 |
| Voltage (kV) | 300 |
| Electron exposure (e– Å−2) | 70 |
| Defocus range (μm) | −1.5 to −2.5 |
| Pixel size (Å) | 0.81 |
| Symmetry imposed | C1 (that is, none) |
| Initial particle images (no.) | 403,566 |
| Final particle images (no.) | 15,954 |
| Map resolution (Å) | 2.9 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2–10 |
| Refinement | |
| Initial model used (PDB accession no.) | 3JCT |
| Model resolution (Å) | 2.9 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | Not applicable |
| Map sharpening B factor (Å2) | 19.0 |
| Model composition | |
| Nonhydrogen atoms | 140,993 |
| Protein residues | 8,739 |
| Nucleotides | 3,338 |
| Ligands | Mg2+: 1 |
| B factors (Å2) | |
| Protein | 38.9 |
| Ligand | 62.99 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.010 |
| Bond angles (°) | 0.880 |
| Validation | |
| MolProbity score | 1.88 |
| Clashscore | 11.12 |
| Poor rotamers (%) | 0.01 |
| Ramachandran plot | |
| Favored (%) | 95.37 |
| Allowed (%) | 4.62 |
| Disallowed (%) | 0.01 |