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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Circ Heart Fail. 2022 Dec 16;16(1):e009972. doi: 10.1161/CIRCHEARTFAILURE.122.009972

Fig. 4. PAG rapidly induces BNP gene expression in vitro and in vivo.

Fig. 4.

(A) Fold-change of BNP (Nppb) gene expression in H9c2 rat cardiomyoblasts, normalized to GAPDH, after 4 h exposure to 100 μM PAGln or PAGly measured in the indicated number of replicates (n=10–12 as indicated). (B) Fold-change of BNP (Nppb) gene expression in isolated left atria, normalized to GAPDH, 15 min after IP injection of PAGln (50 mg/kg), PAGly (50 mg/kg), or vehicle control measured in the indicated number of animals (n=14–16 as indicated). Circulating plasma levels of PAGln and PAGly (mean ± SEM μM) are indicated below the graph. One-way ANOVA followed by Tukey’s multiple comparison test listed. All bars represented the mean ± SEM and dots indicate single data points.