FIG. 5.

Adherence of platelets in PRP to EC (A) and S. aureus wild type to EC treated with PRP (B). (A) [³H]adenine-labeled platelets in autologous citrated plasma (200 μl/well containing 2 × 10⁷ to 4 × 10⁷ cells) treated with PBS–0.1% BSA, 1 μM PMA, or PGE₁/IBMX for 20 min were incubated with EC for 20 min at 37°C under static conditions. After washing and solubilization, the number of adherent platelets was measured. (B) EC monolayer was incubated for 20 min at 37°C under static conditions with PRP containing nonradiolabeled platelets treated with PBS–0.1% BSA, PMA, or PGE₁/IBMX. After washing, [³H]thymidine-labeled wild-type S. aureus cells (2 × 10⁸ CFU) were placed on the EC surface and incubated for 20 min at 37°C under flow conditions. Samples were washed, and the number of adherent bacteria was measured. Data are mean ± SD for three to five determinations performed in duplicate.