1- To grind the fresh horse’s pancreas with sand after the addition of sodium bicarbonate, mixing the product with kieselguhr [*]

2- To apply a pressure of 350 atmospheres in a hydraulic press, collecting the filtrate in a Buchner system

3- To mix the fluid with alcohol and filter off the sediment

4- To evaporate the filtrate at low temperature in a vacuum, add water and neutralize the solution

5- To heat the solution (80 °C), perform a dialysis, filter the content with a sterilized Berkefeld-Donde filter and dilute the final preparation of the extract in 0.45 NaCl