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. 2022 Dec 31;60(2):163–189. doi: 10.1007/s00592-022-02014-7
The glands were extracted during 72 h at 35–40 °C; then filtered and evaporated to dryness and the residue extracted with ether at room temperature. Once discarded the fraction extracted with ether, the residue was dissolved in 95% ethanol. After a new evaporation process, the extract was diluted in saline solution and ready for injection
Scott also prepared watery extracts. After handling the alcoholic extracts as above, the preparation was evaporated to dryness under a vacuum and extracted in absolute alcohol at 38 °C. After pouring off, the residue was extracted with water rendered acid by acetic acid. Then the preparation was filtered and evaporated. Before injecting, the ethanol was eliminated and saline solution added