(…) Paulescu’s researches were communicated at a meeting of the Reunion Roumaine de Biologie in the spring of 1921, when he described the effects produced by intravenous injections of sterile pancreatic extracts on the percentage of sugar, of acetone bodies, and of urea in the blood and urine of depancreatized dogs. There can be no doubt that all three substances became markedly reduced in amount, in blood and urine, as a result of the injections. The results were the same whether the injection was made into a branch of the portal vein or into a yugular vein

(…) The effects were noticeable in one hour following the injection, attain their maximum in two hours, and passed off in twelve hours. They varied with the amount of gland present in the injected extract

Paulescu also observed that the blood sugar, as well as the blood urea, in a normal dog became lowered by the injections

JJR Macleod, 1926