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. 2023 Jan 19;13:67. doi: 10.1038/s41598-022-24529-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of significant physicochemical amino acid changes using TreeSAAP and genes trees. This analysis was performed on the genes that presented higher ω values identified by codeML analysis in giant cetaceans. Only GHSR (A) and IGFBP7 (B) showed significant results. A highly significant z-score (z > 3.09, p < 0.01), represented here by the regions above the orange line, indicates more non-synonymous substitutions than assumed under the neutral model and therefore are interpreted as a result of positive selection. Respective property and category are shown above the graphs.